Multiplex Specific IgE Profiling in Neonatal Stool of Preterms Predicts IgE-Mediated Disease

Background: The natural history of immunoglobulin (Ig) E-mediated diseases in preterm infants is still elusive. We aimed at developing a non-invasive tool for detecting specific IgE (sIgE) and eosinophil-derived neurotoxin (EDN) neonatal fecal samples evaluating its predictive value the development IgE-mediated during first year life. Methods: developed stool extraction protocol, followed by freeze-drying solubilization. sIgEs were investigated from 21 with 300-allergen multiplex confirmed capillary Western blot nano-immunoassay. EDN concentration was used to investigate local eosinophilic component. Results: multiplexed allergen assay detected sIgE all samples. A confirm results. frequency levels differed between who controls. Allergen specificity associated cow’s milk allergy (CMA) asthma. CMA predicted response proteins (sensitivity 88%; 78%). 100%; 75%). Conclusion: investigation promising predicting subsequent diseases. Clinical Implications: profiling can predict